Automated Air-Liquid Interface Cell Culture Analysis Using Deep Optical Flow

Autogenerated Report: RAINBOW Air-Liquid Interface Cell Culture Analysis

Website: https://github.com/AlphonsGwatimba/Automated-Air-Liquid-Interface-Cell-Culture-Analysis-Using-Deep-Optical-Flow

Source directory

C:\Users\Alphons\OneDrive\Documents\examples\example_image_series\(tif)_191018_HNA-ALI_d14.nd2_-_191018_HNA-ALI_d14.nd2_(series_03)0000_etc

Image Series Type

.tif

RAINBOW Analysis Timestamp

26 October 2021, 22:24:30

Image Series Metadata

{
    "analysis_timestamp": "26 October 2021, 22:24:30",
    "calibration_um": 0.31302569743655434,
    "dir": "C:\\Users\\Alphons\\OneDrive\\Documents\\Automated-Air-Liquid-Interface-Cell-Culture-Analysis-Using-Deep-Optical-Flow\\examples\\example_image_series",
    "type": ".tif"
}

Magnitude Statistics

    frame  min (um)  max (um)  mean (um)  std (um)  var (um)
0       0  0.001922  4.178232   2.138720  0.771738  0.595579
1       1  0.001548  3.935667   2.059078  0.799700  0.639520
2       2  0.102884  4.091494   2.039340  0.814543  0.663481
3       3  0.003528  3.479420   1.967105  0.759979  0.577568
4       4  0.004041  3.422691   1.889933  0.741849  0.550339
5       5  0.001328  3.128321   1.746436  0.634321  0.402363
6       6  0.005252  3.086466   1.701845  0.645827  0.417092
7       7  0.004326  3.372862   1.699563  0.644477  0.415350
8       8  0.002674  3.382968   1.754328  0.715596  0.512077
9       9  0.002631  3.494042   1.703855  0.734561  0.539580
10     10  0.003292  3.258859   1.729070  0.700330  0.490462
11     11  0.001937  3.233230   1.721925  0.726743  0.528155
12     12  0.000836  3.261819   1.664737  0.703804  0.495340
13     13  0.001781  3.125801   1.658645  0.718937  0.516871
14     14  0.002709  3.131471   1.644901  0.714538  0.510565
15     15  0.005130  3.323296   1.636717  0.735912  0.541567
16     16  0.001505  3.345543   1.656139  0.709268  0.503061
17     17  0.001450  3.342792   1.668112  0.729482  0.532144
18     18  0.000374  3.507166   1.661115  0.741280  0.549495

Direction Statistics

    frame  min (deg)   max (deg)  mean (deg)   std (deg)   var (deg)
0       0   0.000041  359.999969   43.281398   42.713046   31.841863
1       1   0.000414  359.999176   35.513472   50.172640   43.935066
2       2   0.000021  359.999939   43.784305   50.391486   44.319178
3       3   0.000308  359.999969   36.392191   55.718112   54.183885
4       4   0.000931  359.999969   30.522442   63.856825   71.169188
5       5   0.000555  360.000000   34.993716   81.487165  115.892620
6       6   0.000189  359.999969   42.838759   88.519528  136.758883
7       7   0.000036  359.999969   29.267093   88.033610  135.261558
8       8   0.000118  360.000000   25.796509   83.577514  121.914752
9       9   0.000309  359.999481   18.781744   96.761737  163.412275
10     10   0.000064  359.999908   27.118343   92.455570  149.191310
11     11   0.000063  359.999847   10.934727   95.589849  159.478052
12     12   0.000106  359.999481   19.917667  100.345838  175.742215
13     13   0.000120  359.999817   38.717614   95.348777  158.674677
14     14   0.000547  359.998749   26.982298   95.167638  158.072363
15     15   0.000079  359.999695   45.586196   95.688976  159.808979
16     16   0.000247  359.999451   26.400448   93.869467  153.789283
17     17   0.000003  359.999939   47.943148   86.507419  130.612299
18     18   0.000201  359.999603   42.746710   95.632914  159.621778

Magnitude Scatter Plot

<Figure size 432x288 with 0 Axes>

Direction Scatter Plot

<Figure size 432x288 with 0 Axes>

Raw Image Series (left) and RAINBOW Optical Flow Visualisation (Right)

a) The direction of motion at any position within RAINBOW generated optical flow images is measured clockwise from the initial horizontal position of a unit circle (left) and is shown using hue values (right).

Magnitude Heatmaps Across Image Series

Visualization of motion magnitiude using heatmap with hot colour mapping.

Quiver Plots Across Image Series

Visualisation of optical flow using quiver plots containing vector arrows.